Structural and Functional Similarities between Osmotin from Nicotiana Tabacum Seeds and Human Adiponectin
نویسندگان
چکیده
Osmotin, a plant protein, specifically binds a seven transmembrane domain receptor-like protein to exert its biological activity via a RAS2/cAMP signaling pathway. The receptor protein is encoded in the gene ORE20/PHO36 and the mammalian homolog of PHO36 is a receptor for the human hormone adiponectin (ADIPOR1). Moreover it is known that the osmotin domain I can be overlapped to the β-barrel domain of adiponectin. Therefore, these observations and some already existing structural and biological data open a window on a possible use of the osmotin or of its derivative as adiponectin agonist. We have modelled the three-dimensional structure of the adiponectin trimer (ADIPOQ), and two ADIPOR1 and PHO36 receptors. Moreover, we have also modelled the following complexes: ADIPOQ/ADIPOR1, osmotin/PHO36 and osmotin/ADIPOR1. We have then shown the structural determinants of these interactions and their physico-chemical features and analyzed the related interaction residues involved in the formation of the complexes. The stability of the modelled structures and their complexes was always evaluated and controlled by molecular dynamics. On the basis of these results a 9 residues osmotin peptide was selected and its interaction with ADIPOR1 and PHO36 was modelled and analysed in term of energetic stability by molecular dynamics. To confirm in vivo the molecular modelling data, osmotin has been purified from nicotiana tabacum seeds and its nine residues peptide synthesized. We have used cultured human synovial fibroblasts that respond to adiponectin by increasing the expression of IL-6, TNF-alpha and IL-1beta via ADIPOR1. The biological effect on fibroblasts of osmotin and its peptide derivative has been found similar to that of adiponectin confirming the results found in silico.
منابع مشابه
Osmotin attenuates amyloid beta-induced memory impairment, tau phosphorylation and neurodegeneration in the mouse hippocampus
The pathological hallmarks of Alzheimer's disease (AD) include amyloid beta (Aβ) accumulation, neurofibrillary tangle formation, synaptic dysfunction and neuronal loss. In this study, we investigated the neuroprotection of novel osmotin, a plant protein extracted from Nicotiana tabacum that has been considered to be a homolog of mammalian adiponectin. Here, we observed that treatment with osmot...
متن کاملOsmotin gene expression is posttranscriptionally regulated.
Accumulation of both osmotin mRNA and osmotin protein in tissues of tobacco (Nicotiana tabacum L. var Wisconsin 38) is subject to complex developmental control. Osmotin was found to be most abundant in tobacco roots and in tissues of the outer stem comprised primarily of epidermis, and it was less abundant in the corolla. It was a minor protein in other tissues and was undetectable in some tiss...
متن کاملStable NaCl Tolerance of Tobacco Cells Is Associated with Enhanced Accumulation of Osmotin.
Osmotin is a major protein which accumulates in tobacco cells (Nicotiana tabacum L. var Wisconsin 38) adapted to low water potentials. Quantitation of osmotin levels by immunoblots indicated that cells adapted to 428 millimolar NaCl contained 4 to 30 times the level of osmotin found in unadapted cells, depending on the stage of growth. Unadapted cells accumulated low levels of osmotin with appa...
متن کاملSeed legumains are expressed in stamens and vegetative legumains in seeds of Nicotiana tabacum L.
Detailed analysis of the expression pattern of seven legumain genes from Nicotiana tabacum L. cv. SNN revealed that it showed neither coincidences with the branches of the phylogenetic tree of legumains nor with their conventional assignment to organs. This agreed well with the fact that, so far, no functional differences could be assigned to the sequence differences reflected in the branches o...
متن کامل